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Culture And Characterization Of Cancer Stem Cells Derived From Glioblastoma Multiforme Tumors

The most typical kind of cancer that may occur in the brain is called glioblastoma multiforme. The modern theory of how it develops is centered on cancer stem cells, which are thought to be transformed versions of normal neural stem cells that are already present in the brain.

Alexander McCaslin
Dec 05, 202213 Shares664 Views
The most typical kind of cancer that may occur in the brain is called glioblastoma multiforme. The modern theory of how it develops is centered on cancer stem cells, which are thought to be transformed versions of normal neural stem cells that are already present in the brain.
Markers such as Nestin, Sox-2, CD133, CD44, and sometimes glial fibrillary acidic protein are expressed by neural stem cells and cancer stem cells alike. Both kinds of cells have comparable signal transduction pathways as well as a variety of features including the ability to differentiate and proliferate.
Self-renewal is another shared trait. The purpose of this study is to examine the various strategies for cultivating cells extracted from glioblastoma multiforme as well as the expression of cancer stem cell markers that correlate with those strategies.

Neural Stem Cells

From the developing embryo to the fully mature organism, neural stem cells have been characterized and localized to the hippocampus's subgranular zone, dentate gyrus, and ventricular zone. Neural stem cells congregate in these niches near blood arteries.
Pluripotent cells with the ability to self-renew and differentiate, such as neural stem cells, lose their "stemness" upon differentiation. The factors responsible for keeping neural stem cells in an undifferentiated state, epidermal growth factor and basic fibroblast growth factor, must be absent from the blood serum for this to occur.
Microfilament protein nestin (Nestin) is located in the cytoplasm and has a role in cytoskeleton organization, cell signaling, organogenesis, and cellular metabolism.

Cancer Stem Cells

Organised chaos describes the cellular makeup of glioblastoma multiforme, which includes pleomorphic tumor cells, growing blood vessels, invading inflammatory cells, and necrosis. These terminally differentiated cells, known as cancer stem cells, have many characteristics with neural stem cells, including the capacity to develop into neurons and glial cells in the absence of epidermal growth factor and basic fibroblast growth factor, as well as a high proliferation potential controlled by these factors.
The PTET (tumor suppressor gene) mutations are also discussed. These cells seem to be at the pinnacle of the tumor cell hierarchy and may give rise to a glioblastoma multiforme on their own. Both Nestin and glial fibrillary acidic protein are expressed by cancer stem cells, however Nestin is more strongly expressed.
Sox-2 expression is common, but typically displays abnormal expression, indicating a jumbled differentiation process. Furthermore, other markers, including as Musashi-1, CXCR4, and CD15, have been identified in both neural stem cells and cancer stem cells.

The Cancer Stem Cell Theory

People Aslo Ask

What Is The Difference Between Cancer Cells And Cancer Stem Cells?

In clinical terms, the cancer cell lays the groundwork for a developing tumor. On the other hand, cancer stem cells (CSCs) are the cells responsible for continuing tumor growth.

Where Are Cancer Stem Cells Located?

In the case of leukemia, cancer stem cells were initially found. Breast cancer stem cells were the first cancer stem cells to be found in solid tumors, according to research at the University of Michigan. Tumors of the colon, head and neck, pancreas, and central nervous system have all been shown to contain cancer stem cells since then.

How Do Cancer Stem Cells Cause Cancer?

Since stem cells can persist for much longer than typical cells, they have more time to pick up harmful mutations. It may only take a few mutations for a single cell to lose control of its own self-renewal and growth, making it the potential source of cancer.

Final Words

When it comes to primary brain tumors, glioblastoma multiforme is both the most frequent and the deadliest. The theory that normal neural stem cells undergo a metamorphosis into cancer stem cellcell ,s is now regarded as one of the fundamental pathologic elements contributing to the development of Glioblastoma multiforme.
Glioblastoma cells may be cultivated in serum-free medium with epidermal growth factor and fibroblast growth factor, or in media with foetal bovine serum. Combining these fundamental methods allows for media-based cell culture.
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